The Impact of Simulated Microgravity on Mice With Breast Cancer

Alemi, Morteza; Samadi, Firooz; Madjid Ansari, Alireza; Hajebrahimi, Zahra

doi:10.22034/jsst.2024.1494

The Impact of Simulated Microgravity on Mice With Breast Cancer

Document Type : Original Research Paper

Authors

Morteza Alemi ¹

Firooz Samadi ²

Alireza Madjid Ansari ³

Zahra Hajebrahimi ⁴

¹ Ph.D. Student, Department of Animal and Poultry Physiology, Breeding and Genetics, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

² Professor, Department of Animal and Poultry Physiology, Breeding and Genetics, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

³ Assistant Professor, Quality of Life Research Group, Department of Integrative Oncology and Quality of Life, Motamed Cancer Institute Breast Cancer Research Center, ACECR, Tehran, Iran

⁴ Associate Professor, Aerospace Research Institute, Ministry of Science, Research and Technology, Tehran, Iran

10.22034/jsst.2024.1494

Abstract

Exposure to microgravity induces significant changes in the morphology and biological properties of cancer cells. Globally, breast cancer is the most prevalent cancer among women and the fifth leading cause of cancer-related deaths. Increasing resistance to conventional cancer treatments has emerged as a critical challenge, prompting researchers to explore novel anti-cancer strategies to enhance cancer cell death. This study aimed to evaluate the response of breast cancer cells to simulated microgravity. In this experiment, 20 mice carrying the 4T1 breast cancer cell line were randomly divided into groups of 10. The experimental groups included a normal gravity condition and a simulated microgravity condition. Mice were tested over a 30-day period, during which tumor growth was measured every three days using a digital caliper. The number of casualties was recorded daily. At the end of the experiment, the mice were sacrificed, and the final tumor size was measured. The results demonstrated that simulated microgravity significantly inhibited tumor growth over the 30 days compared to the control group under normal gravity. Furthermore, the group exposed to simulated microgravity exhibited the highest survival rate, whereas the control group recorded the lowest. These findings suggest that simulated microgravity effectively reduces tumor progression and enhances survival in mice with breast cancer. Investigating cancer under microgravity conditions may offer valuable insights into the mechanisms underlying tumor progression and resistance, potentially leading to the development of novel treatment approaches.

Keywords

Breast Cancer

Simulated Microgravity: 4T1 Cell Line

Mice

Tumor Size

Subjects

physiology and space medicine (astrobiology)

Article Title Persian

تاثیر بی‌وزنی شبیه سازی شده بر روی موش‌های مبتلا به سرطان پستان

Authors Persian

مرتضی عالمی ¹

فیروز صمدی ²

علیرضا مجید انصاری ³

زهرا حاج ابراهیمی ⁴

¹ دانشجوی دکتری، گروه ژنتیک، اصلاح و فیزیولوژی دام و طیور، دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گلستان، ایران

² استاد، گروه ژنتیک، اصلاح و فیزیولوژی دام و طیور، دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گلستان، ایران

³ استادیار، گروه پژوهشی طب فراگیر در سرطان، دپارتمان کیفیت زندگی و طب فراگیر، پژوهشکده معتمد جهاد دانشگاهی، تهران، ایران

⁴ دانشیار، پژوهشگاه هوافضا، وزارت علوم، تحقیقات و فناوری، تهران، ایران

Abstract Persian

سلول‌های سرطانی زمانی که در معرض بی‌وزنی قرار می گیرند، دستخوش تغییرات چشمگیری در مورفولوژی و خواص بیولوژیکی می‌شوند. سرطان در زنان و پنجمین عامل مرگ و میر ناشی از سرطان در دنیا است. با توجه به اینکه افزایش مقاومت سرطان‌ها نسبت به درمان‌های رایج به مسئله دردسرسازی تبدیل شده است، تلاش محققان برای کشف و شناسایی عوامل ضد سرطانی جدید که موجب افزایش میزان مرگ سلول‌های سرطانی گردد، رو به افزایش است. هدف از این مطالعه بررسی پاسخ سلول‌های سرطانی پستان به شرایط بی‌وزنی بود. در مطالعه حاضر 20 موش حامل سرطان پستان رده سلولی 4T1 در دو گروه که هر گروه آن ده موش در آن قرار داده شده بود تقسیم شدند. گروه‌های آزمایشی شامل شرایط نرمال جاذبه (گروه شاهد) و بی‌وزنی شبیه سازی شده (بی‌وزنی) بودند که موش‌ها به مدت 30 روز تحت آزمایش قرار گرفتند. در طی دوره آزمایش روند رشد تومور هر 3 روز یک بار به وسیله کولیس دیجیتال مورد اندازه‌گیری قرار گرفت. همچنین در طی دوره آزمایش میزان تلفات شمارش می‌گردید. در پایان دوره، بعد از کشتن موش‌ها سایز نهایی تومور اندازه‌گیری شد. نتایج این آزمایش نشان داد که القا شرایط بی‌وزنی به طور معنی داری می‌تواند رشد تومور را بعد از 30 روز کاهش دهد. همچنین القا بی‌وزنی روند رشد تومور را نسبت به گروه شاهد کاهش داد. در انتهای دوره آزمایش، موش‌های گروه القا بی‌وزنی شبیه سازی شده نسبت به گروه شاهد بیشترین زنده‌مانی را نشان دادند. کمترین تعداد موش‌های زنده در گروه شاهد مشاهده شد. در نتیجه می‌توان نتیجه گرفت که القا بی‌وزنی می‌تواند منجر به کاهش رشد تومور و افزایش زنده‌مانی شود. مطالعه سرطان در شرایط بی‌وزنی ممکن است به ما در فهم بهتر مکانیسم درمان این بیماری و شاهد بهتر این بیماری کمک نموده و به توسعه روش‌های درمانی جدید کمک شایانی ‌نماید.

Keywords Persian

سرطان پستان

بی‌وزنی شبیه سازی شده

رده سلولی 4T1: موش

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