E-cadherin Gene Expression Changes in MCF-7 Breast Cancer Cell Line Under Simulated Microgravity Condition

Hajebrahimi, Zahra

doi:10.22034/jsst.2023.1468

E-cadherin Gene Expression Changes in MCF-7 Breast Cancer Cell Line Under Simulated Microgravity Condition

Document Type : Original Research Paper

Author

Zahra Hajebrahimi

Associate Professor, Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iran

10.22034/jsst.2023.1468

Abstract

Breast cancer is the most common type of malignancy in women. Metastasis and invasion of breast tumor cells are responsible for many deaths from breast cancer. Simulated microgravity can alter tumor cell adhesion, proliferation, invasion, and metastasis. The degradation and regeneration of the extracellular matrix play essential roles in the migration and metastasis of tumor cells. E-cadherin is a cell adhesion molecule whose primary function is to mediate cell-matrix and cell-cell contacts, maintaining the integrity of the extracellular matrix. Loss of E-cadherin is one of the initial events in metastasis and tumor invasion.In the present study, the expression of the E-cadherin gene in the MCF-7 breast cancer cell line was examined under simulated microgravity conditions using a clinostat for one and three days, utilizing real-time PCR with GAPDH as a reference gene. The effects of weightlessness on cell proliferation and metabolic activity were evaluated using the MTT assay. MTT data indicated that microgravity for one and three days had no significant effects on cell proliferation and metabolic activity. However, exposure to microgravity for one day led to a doubling of E-cadherin gene expression. Continued exposure to microgravity for three days resulted in a further increase.These findings suggest that microgravity conditions may reduce the invasive phenotype of breast cancer, potentially decreasing breast cancer invasion and metastasis. Studying cancer cells in a microgravity environment can enhance our understanding of the mechanisms underlying cancer progression and may lead to the development of new treatment methods.

Keywords

Simulated Microgravity: Clinostat: Breast Cancer: MCF

7 Cell Line: E

Cadherin Gene

Subjects

cellular-molecular biology

Article Title Persian

تغییرات بیانی ژن E-cadherin در رده سلولی MCF-7 سرطان پستان در شرایط بی‌وزنی شبیه‌سازی‌شده

Author Persian

زهرا حاج‌ابراهیمی

دانشیار پژوهشگاه هوافضا، وزارت علوم تحقیقات و فناوری، تهران،ایران

Abstract Persian

سرطان پستان شایع‌ترین نوع بدخیمی در زنان می‌باشد. متاستاز و تهاجم سلول‌های تومور پستان مسئول بسیاری از مرگ‌های ناشی از سرطان پستان هستند. بی‌وزنی شبیه‌سازی‌شده میتواند چسبندگی سلول‌های توموری، تکثیر، تهاجم و متاستاز آنها را تغییر دهد. تخریب و بازسازی ماتریکس خارج سلولی نقش مهمی در مهاجرت و متاستاز سلول های تومور ایفا میکند. پروتئین E-cadherin یک مولکول چسبندگی سلولی است که وظیفه اصلی آن واسطه‌گری ماتریکس سلولی و تماس‌های سلولی است و منجر به نگهداری و یکپارچگی ماتریکس خارج سلولی می‌شود. از دست دادن E-cadherin یکی از اولین اتفاقات در شروع متاستاز و تهاجم تومور است. درمطالعه حاضر بیان ژن E-cadherin در رده سلولی MCF-7 سرطان پستان در شرایط بیوزنی شبیه سازی شده با دستگاه کلینواستت برای یک و سه روز توسط تکنیک real-time PCR و با ژن مرجع GAPDH مطالعه شد. همچنین تاثیر بی وزنی بر تکثیر و فعالیت متابولیکی سلول با تست MTT ارزیابی شد. داده های MTT نشان داد که بی وزنی برای یک و سه روز تاثیر معناداری بر تکثیر و فعالیت متابولیکی سلول ها ندارد. همچنین بی وزنی برای یک روز منجر به افزایش دوبرابری بیان ژن E-cadherin گردید. ادامه یافتن بیوزنی برای سه روز، منجر به افزایش بیشتر آن گردید. درنتیجه استفاده از شرایط بی وزنی ممکن است ازاین طریق منجر به کاهش فنوتیپ سرطانی پستان و کاهش تهاجم و متاستاز سرطان پستان گردد. بنابراین مطالعه سلول‌های سرطانی در محیط بی‌وزنی می‌تواند به ما در فهم مکانیسم‌های که منجر به ایجاد سرطان و پیشرفت آن می شود کمک کند. همچنین این مطالعات می تواند موجب توسعه روش‌های درمانی جدید برای درمان سرطان گردد.

Keywords Persian

بی وزنی شبیه سازی شده

کلینواستت

سرطان پستان

رده سلولی MCF-7

ژن E-cadherin

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