Journal of Space Science and Technology

Journal of Space Science and Technology

The Study of Hypergravity and Flight Stress on Different Cell Lines Function in Sounding Rocket

Authors
Abstract
Stress environment of space flights including hypergravity and microgravity effect cell functions and processes. We studied effect of hypergravity and flight stress on function, death, growth and cellular damage of five cell lines including human vascular endothelial cells, rat bone marrow stromal cells, mouse embryonic fibroblast cells, PC12 cells and pancreas islands in Biological payload of 3th sounding rocket by NO, LDH, MTT and insulin assay kits. Our results indicated that effect of hypergravity increase significantly cellular death and NO secretion but we found no changes in insulin secretion and pancreas function.
Keywords

Article Title Persian

بررسی اثرات ناشی از هایپرگراویتی و استرس پرتاب بر عملکرد رده‌های سلولی مختلف در کاوشگر

Authors Persian

زهرا حاج‌ابراهیمی
لیلا علیدوست
مائده عربیان
احسان علوی
محمد ابراهیمی
محسن بهرامی
Abstract Persian

شرایط استرس‌زای پرتاب باکاوشگرها - هایپرگراویتی و جاذبة ناچیز- منجر به تغییر در فرایندها و عملکردهای سلولی می‌شود. در این مطالعه، اثرات هایپرگراویتی و استرس ناشی از آن بر روی میزان فعالیت، رشد، مرگ و میر و آسیب‌ سلولی در 5 ردة سلولی اندوتلیال عروق انسان، سلول‌های بنیادی مغز استخوان موش صحرایی، فیبروبلاست جنینی موش، PC12و جزایر لانگرهانس موش صحرایی از طریق سنجش میزان NO، LDH، MTTو انسولین توسط ماژول کپسول زیستی کاوشگر 3 انجام شد.نتایج نشان داد که هایپرگراویتی موجب افزایش معنی‌دار در ترشح NOو مرگ ‌و میر سلولی می‌شود اما تغییری در عملکرد پانکراس در تولید انسولین مشاهده نشد.

Keywords Persian

استرس
کاوشگر
لاکتات دهیدروژناز
نیتریک اکسید
هایپرگراویتی
[1] Clement, G. and Slenzka, K., Fundamentals of Space Biology Research on Cells, Animals, and Plant in Space, Springer, 2006.
[2] Takaoki, M., et al., “Development and Operation of Cultured Cell Experiment with TR-IA Sounding Rocket,” Biological Science Space, Vol. 12, No. 3, 1998, pp. 320-321.
[3] Gurkin, L. W., “The NASA Sounding Rocket Program and Space Sciences,” ASGSB Bulletin, Vol. 6, No. 1, 1992, pp. 113-120.
[4] Jongkind, J. F., Visser, P. and Verkerk, A., Somatic Cell Fusion During Sounding Rocket Flight. Life Sciences Experiments Performed on Sounding Rockets (1985-1994): European Space Agency, ESA SP-1206, ISBN: 92-9092-423-3, 1997, p. 99.
[5] Freshney, R. I., Culture of Animal Cells: A Manual of Basic Technique, 5th Edition, Wilely, 2005.
[6] Hansen, W. A. and et al., “Supravital Dithizone Staining in the Isolation of Human and Rat Pancreatic Islets,” Diabetes Res., Vol. 10, No. 2, 1989, pp.53-7.
 [7] Mirand, K. M., Espey, M. G. and Wink, D. A., “A Rapid, Simple Spectrophotometric Method for Simultaneous Detection of Nitrate and Nitrite,” Nitric Oxide, Vol. 5, No.1, 2001, pp. 62-71.
[8] Legrand, C., et. Al., “Lactate Dehydrogenase (LDH) Activity of the Number of Dead Cells in the Medium of Cultured Eukaryotic Cells as Marker,” Journal of Biotechnology, Vol. 25, No. 3, 1992, pp. 231–243.
[9] Matsuoka, M., “Cell Proliferation and Cytotoxicity Assay. Biochem,” Pharmacol, Vol. 59, 2000, p. 1573.
[10] Space Studies Board, National Reaserch Council, A Strategy for Research in Space Biology and Medicine in the New Century, National Academy Press, Washington, D. C., 1998.
[11] Sun, J., Zhang, X., Broderick, M. and Fein, H., “Measurement of Nitric Oxide Production in Biological Systems by Using Griess Reaction Assay,” Sensors, Vol. 3, No. 8, 2003, pp. 276-84.
[13] Blaber, A. P., Goswami, N., Bondar, R. L., Kassam, M. S., “Impairment of Cerebral Blood Flow Regulation in Astronauts with Orthostatic Intolerance after Flight,” Stroke. Vol. 42, No. 7, 2011, pp. 1844-50.
[14] Strollo, F., “Hormonal Changes in Humans During Spaceflight,” Advances Space Biology and Medicine, Vol.7, 1999, pp. 99-129.
Volume 6, Issue 4 - Serial Number 17
شماره پیاپی 17
Winter 2014
Pages 37-42

  • Receive Date 21 May 2014
  • Revise Date 05 February 2024
  • Accept Date 19 April 2016
  • First Publish Date 19 April 2016